Dielectrophoretic isolation of DNA and nanoparticles from blood cancer cell

http://biologicaldynamics.com/research/dielectrophoretic-isolation-of-dna-and-nanoparticles-from-blood/

Dielectrophoretic isolation of DNA and nanoparticles from blood

http://www.forbes.com/pictures/mkg45giif/raj-krishnan-chief-executive-biological-dynamics-inc-29/

http://www.tedx-sandiego.com/speaker/raj-krishnan/

Raj Krishnan, Chief Executive, Biological Dynamics, Inc., 29

Developing blood tests that use electric fields to detect key signals that a patient has cancer from the blood.

The ability to effectively detect disease-related DNA biomarkers and drug delivery nanoparticles directly in blood is a major challenge for viable diagnostics and therapy monitoring. A DEP method has been developed which allows the rapid isolation, concentration and detection of DNA and nanoparticles directly from human and rat whole blood.

Biological Dynamics Inc. is interested in partnership opportunities with Pharmaceutical, Life Science Tool companies and research organizations.

Biological Dynamics has a fully functional research laboratory capable of handling fresh blood, serum and plasma as well as frozen serum and plasma samples as part of material transfer agreements. We are seeking partners willing to explore the potential of our AC Electrokinetics technology in the areas of oncology, sample preparation and novel applications. Please contact us here.

http://onlinelibrary.wiley.com/doi/10.1002/elps.201100700/abstract

The ability to effectively detect disease-related DNA biomarkers and drug delivery nanoparticles directly in blood is a major challenge for viable diagnostics and therapy monitoring. A DEP method has been developed which allows the rapid isolation, concentration and detection of DNA and nanoparticles directly from human and rat whole blood. Using a microarray device operating at 20 V peak-to-peak and 10 kHz, a wide range of high molecular weight (HMW)-DNA and nanoparticles were concentrated into high-field regions by positive DEP, while the blood cells were concentrated into the low-field regions by negative DEP. A simple fluidic wash removes the blood cells while the DNA and nanoparticles remain concentrated in the DEP high-field regions where they can be detected by fluorescence. HMW-DNA could be detected at 260 ng/mL, which is a detection level suitable for analysis of disease-related cell-free circulating DNA biomarkers. Fluorescent 40 nm nanoparticles could be detected at 9.5 × 10⁹ particles/mL, which is a level suitable for monitoring drug delivery nanoparticles. The ability to rapidly isolate and detect DNA biomarkers and nanoparticles from undiluted whole blood will benefit many diagnostic applications by significantly reducing sample preparation time and complexity.

Investor Relations

Biological Dynamics Incorporated is a privately held company. We have recently closed our Series B round.

If you are a current investor and have a question for Biological Dynamics, or if you are interested in future opportunities to invest in our company, please contact us here.

Biological Dynamics Inc. is an early stage company that has developed a proprietary AC Electrokinetic technology to rapidly extract nanoparticles in high-conductance physiological solutions. Biological Dynamics was founded in 2008 by Raj Krishnan and David Charlot, Bioengineering PHD graduates from the University of California, San Diego (UCSD). Our vision is to enable healthcare practitioners to detect, diagnose and monitor cancer and other diseases from a simple blood draw (or other bodily fluid) and rapidly intervene, increasing patient survivability while driving down the total burden costs.

Raj Krishnan graduated with a B.S. in Electrical Engineering from UCLA in 2004, and received an M.S. and Ph.D. in Bioengineering from UCSD in 2010.  In 2009, Raj won the UCSD $80,000 Entrepreneur Challenge, was a finalist in $250,000 DFJ-Cisco Global Business Plan Competition and won the “Best Pitch” Award in 3rd annual TCA Quick Pitch Competition. He co-founded and is CEO of Biological Dynamics, Inc., a spin-off company based off of technology developed during his Ph.D. on identifying cancer biomarkers from blood using AC Electric Fields. In 2012, Raj was named one of Forbes Magazine 30 under 30: Rising stars of Science.

The Biological Dynamics AC Electrokinetic (ACE) implementation rapidly isolates (<15 min) and quantifies a variety of nanoparticles in bodily fluids without sample pretreatment. ACE allows the ability to preferentially extract particles of certain size threshold, namely 10nm-800nm in diameter, including Cell Free Nucleic Acids, Cellular Membrane Fragments, Exosomes, Large Protein Complexes and Viruses.

The heart of our ACE system is our proprietary microlectrode array chip, specifically designed to selectively capture nanosomes at certain size thresholds. The system is designed for single patient or single test use. Currently our systems are for internal use only but we are currently evaluating an external version for collaboration with research partners